, for example, shows retention times for four weak acids in two mobile phases with practically equivalent values for (P^ key ). Even though the order of elution is similar for both equally mobile phases, Just about every solute’s retention time is impacted in different ways by the selection of natural and organic solvent.The goal is for every one
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-hydroxybenzoic acid—on a nonpolar C18 column utilizing a aqueous mobile phase that features a buffer of acetic acid and sodium acetate. Retention periods are shorter for less acidic mobile phases for the reason that Just about every solute is existing within an anionic, weak foundation kind that is certainly much less soluble during the nonpolar
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